B (1-3 months) Developed by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J. 自治医科大学 B（1〜3か月） 鈴木　誠 <a href='https://brc.riken.jp/mus/pcr01939'>Genotyping protocol -PCR-</a> TRPV4遺伝子のノックアウトマウス。Trpv4 (transient receptor potential cation channel, subfamily V, member 4) は機械刺激や温度を感受してCa<2+>を通すチャネルである。ホモノックアウトマウスは、生存性、妊性ともに正常であるが、浸透圧感受、圧感受、温度感受の低下や、すり応力、痛みや刺激の反応低下などが、様々な臓器で報告されている。 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). RBRC01939 B6.129X1-Trpv4<tm1Msz> B6.129X1-Trpv4<tm1Msz> Heterozygote x Wild-type [C57BL/6JJcl] Heterozygote x Wild-type [C57BL/6JJcl] In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J, Biol. Chem., 278, 22664-22668 (2003). Am. J. Physiol. Cell Physiol., 285, C96-C101(2003). RW4 [129X1/SvJ] Makoto SUZUKI mouse Trpv4 genomic DNA, PGK-neo cassette (mouse phosphoglycerate kinase-1 promoter, E. coli aminoglycoside phosphotransferase cDNA)derived from the plasmid pKJ2. 自治医科大学・水野　誠先生（2001）。RW4 ES細胞を用いて作出。C57BL/6へ戻し交配された。 Jichi Med. Sch. TRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation. Sensorineural Research TRPV4遺伝子のノックアウトマウス。Trpv4 (transient receptor potential cation channel, subfamily V, member 4) は機械刺激や温度を感受してCa<2+>を通すチャネルである。ホモノックアウトマウスは、生存性、妊性ともに正常であるが、浸透圧感受、圧感受、温度感受の低下や、すり応力、痛みや刺激の反応低下などが、様々な臓器で報告されている。, Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> true TRPV4KO TRPV4KO